a) In alkaline lysis method, centrifuge is used to obtain cells from bacterial culture. In which other steps would you use centrifugation? What components are separated after centrifugation? (10 Points) b) You isolated plasmid DNA from E. coli. You will quantify DNA from the liquid in Eppendorf using the spectral Wethod. But before you quantify the amount of DNA in the liquid, you remembered that you didn't use potassium acetate when you did the experiment. In this case, when you make a spectral reading, what can be the value of the results? Explain why (10 Points)