A protein "A" binds to a ligand L with a Kd of 0.4M. a) Calculate the concentration of ligand ([L]) when the fraction of occupied sites () is 0.25,0.6, 0.75 and 0.9 and complete the table below: b) Draw the curve of fraction of occupied sites () vs ligand concentration ([L]) that describes the values provided above ( Kd, and values in the table). What kind of shape does this curve have (or in other words, what mathematical function describes it)? c) Define Kd in thermodynamic terms and in terms of binding site occupancy. d) What does it mean for the protein to be "saturated", and in which region of the binding curve do we observe saturation? e) In the graph you drew for b, draw a second curve for a protein " B " that binds the ligand L with an affinity of 100nM (use a dashed line or different color). Which protein binds to the ligand with higher affinity