Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

A reversed phase HPLC separation is performed with a C18 column using H2O methanol 60:40%v/v as mobile phase. Dimensions of the column are given: 150mm

image text in transcribed
A reversed phase HPLC separation is performed with a C18 column using H2O methanol 60:40%v/v as mobile phase. Dimensions of the column are given: 150mm (length) and 4.6mm (diameter). The particle size of the packing material is 5m(dp). The analyst switched to another C18 column with column dimensions of 100 mm (length) and 4.6mm (diameter) with a 3.5m(dp) particle size packing. The flow rate and mobile phase composition remained the same. (i) Predict the effect of changing to a column with 3.5m particle size packing, in terms of the effect on the run-time. (ii) Estimate the % change in the pressure, given P=kdp2

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image_2

Step: 3

blur-text-image_3

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Fundamentals Of General Organic And Biological Chemistry

Authors: John E. McMurry, Carl A. Hoeger, Virginia E. Peterson, David S. Ballantine

7th Edition

978-0321750839, 0321750837

More Books

Students also viewed these Chemistry questions

Question

Beveridge countries typically feature single-payer insurance.

Answered: 1 week ago

Question

Which state did Dillards pay the most sales tax in?

Answered: 1 week ago

Question

How much sales tax did Dillards pay in New Mexico (NM)?

Answered: 1 week ago