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( a ) The chromatogram below shows a Reversed - Phase Liquid chromatography ( RPLC ) gradient to separate a mixture over 4 0 minutes.

(a) The chromatogram below shows a Reversed-Phase Liquid chromatography (RPLC)
gradient to separate a mixture over 40 minutes. The mobile phase consisted of
H2O? Acetonitrile (5-95%).
All peaks eluted between 17.2min and 34.7min.
(i) Using the chromatographic details given above, determine if isocratic or gradient
elution is more suitable for the separation of this mixture.
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