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-Amylase from malt is used to hydrolyze starch. The dependence of the initial reaction rate on temperature is determined experimentally. Results measured at fixed starch
-Amylase from malt is used to hydrolyze starch. The dependence of the initial reaction rate on temperature is determined experimentally. Results measured at fixed starch and enzyme concentrations are listed in the following table. a) Determine the activation energy for this reaction (kJ/mol) b) -Amylase is used to break down starch in baby food. It is proposed to conduct the reaction at a relatively high temperature so that the viscosity is reduced. What is the reaction rate at 55C compared to 25C ? c) Thermal deactivation of this enzyme is described by the equation kd=2.251027e41,630/RT Where kd is the deactivation rate constant in h1,R is the ideal gas constant in calgmol1K1, and T is the temperature in K. What is the half-life of the enzyme at 55C compared with 25C ? Which of these two operating temperatures is more practical for processing baby food? Explain your answer. Note: The half-life can be calculated from: t1/2=kdLn2
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