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An antibody with two equivalent binding sites is present at a concentration of 2 0 nM . If an antigen is present at 4 0
An antibody with two equivalent binding sites is present at a concentration of nM If an
antigen is present at nM and the KD for binding either end is nM what fraction of antibody is singly
bound at steady state? What fraction is doubly bound at steady state? Assume antigen is in excess
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