Question
An investigator plots 1/ v as a function of 1/(concentration of substrate) for data collected with and without an inhibitor. The best-fit line of the
An investigator plots 1/v as a function of 1/(concentration of substrate) for data collected with and without an inhibitor. The best-fit line of the data collected in the absence of the inhibitor had a slope of 0.22 (arbitrary units of time) and a y-intercept of 0.04 (arbitrary units of time per concentration). The best-fit line of the data collected in the presence of the inhibitor had a slope that was 2.9-times as steep (arbitrary units of time) and a y-intercept of 0.04 (arbitrary units of time per concentration). If the concentration of inhibitor was 79 nM, what is the value of the inhibition constant Ki in terms of nM to the nearest tenth?
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