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Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K, = 5.01 x 109 and pK, =

Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K, = 5.01 x 109 and pK, = 8.3) %3D (a) Calculate the pH of the Tris buffer after mixing 11.0 mL of 0.20 M HCI solution with 25.0 mL of 0.10 M Tris. (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00019 mol of H* was produced. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?

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