biochemistry -bioinorganic

4. A sample of a rubredoxin protein was purified and analyzed by UV-vis spectroscopy (red spectrum). The metal was removed from the protein to produce the apo form (black spectrum). Then, the researchers reconstituted the metal center and took a third spectrum (blue). Answer the following questions about this experiment and the resulting data: a. At what wavelengths are peaks observed in the spectrum of the protein as originally purified? b. For each wavelength you listed, what is its extinction coefficient? c. At what wavelengths are peaks observed in the spectrum of the apo protein? d. Oxidized rubredoxins typically have ligand-to-metal charge transfer (LMCT) bands at 356nm,386nm,494nm,578nm, and 760nm. Is the protein as-purified in the oxidized or reduced state? e. Based on your answer to part (c) above, what oxidation state do you expect the Fe to be in? f. Based on these data, did the researchers successfully reconstitute the metal center