Bioprocess Engineering

3.18. Two enzymes are both immobilized on the same flat, nonporous surface. For enzyme A the sub- strate is S. For enzyme B the substrate is S. The product of the first reaction is S. That is SS- a. Figure 3.P1 depicts the rate of the first reaction on the surface as a function of local com centrations of S If the bulk concentration of Sis 100 mg/l and the mass transfer coeffi- cient is 4 x 10 cm/s, what is the rate of consumption of S, for a 1 cm surface? What is the surface concentration of 2 1. The rate of the second reaction is: - d[S, Vir d[Pd K+S 102 Enzymes Chap 3 40 30H V or J X106 20 mg cm2-5 10F 20 40 100 60 80 S, (mg/l) Figure 3Pl. Reaction rate data for prob 120 lem 3.18. Reaction rate dependence on sub strate one for reaction catalyzed by Ex where K.-5mgl (or 5 x 10 mg/cm) and V." -4,0x10 mg/cm* =s. The bulk concentrs tion of S, (S) is maintained as 5 mg/l and the mass transfer coefficient is the same for S and S. Calculate (Sara) and the rate of formation of P(assuming all stoichiometric coeffi- cients are one)