Bioremediation was selected to clean up 1000 m3 soil contaminated with 1000 mg/kg n-nonane (C9H20), 1000 mg/kg toluene (C7H8), and 2000 mg/kg benzene 1,2-diol (C6H6O2). The soil bulk density is 1,800 kg/m3.

(A) Estimate the average chemical structure/molecular formula of the contaminants (20%)

(B) Determine the most appropriate electron acceptor and metabolism mode for destruction of the above hydrocarbon mixture (20%)

(C) Estimate the amount (kg) of electron acceptor (20%) and nutrient (nitrogen (20%) and phosphorous (20%)) needs Assumptions:

Ammonia is used as the nitrogen source;

Phosphorus need for microbial growth is approximately one-sixth of that for nitrogen source;

The composition of cellular structure is C5H7O2N.

The energy factors for the conversion of contaminants to biomass are listed in the following table:

Electron acceptor	fs value
O2	0.5
NO3	0.3
SO4	0.1
CO2	0.2

** Please do not copy other submitted work **