Cellulase using DNS assay Incubation time: 30 minutes Enzyme sample: 0.5mL Glucose (molecular weight): 180.156g/mol For example: The standard curve of glucose: y=3.4677x (Absorbance 540nm ) reading for 4th hour =0.034 Concentration of glucose =0.034/3.4677 =0.009804mg/mL Concentration of protein (Absorbance 595nm ) =28.12mg/mL Questions: 1. How to convert mg/mL to mol unit? (Show the calculation step by step) The quantity of glucose produced in mol ? 2. Determine the enzyme activity ( mol/mL/min) and specific activity ( mol/min/mg). (Show the calculation step by step)