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Consider a batch reactor where the substrate lactose is being enzymatically hydrolyzed by galactosidase (MW=540,000) by the following reaction: Lactose +H2O glucose + galactose It
Consider a batch reactor where the substrate lactose is being enzymatically hydrolyzed by galactosidase (MW=540,000) by the following reaction: Lactose +H2O glucose + galactose It has been determined that the enzyme catalyzed reaction follows Michaelis-Menten kinetics. The reaction rate expression for Michaelis-Menten kinetics is given by: dtd[Lactose]=Km+[Lactose]Vmax[Lactose] Experiments are carried out to determine the kinetic parameters of the enzyme. The initial rate data are as follows. Use non-linear regression to determine the Vmax and Km for the enzyme. Determine the kcat of the enzyme assuming the reaction was performed at a concentration of 50mg/L of enzyme
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