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Determine the optimal incubation condition for HaCat cells to grow? Answer all questions and record all calculations in your lab book. 1. Calculate the appropriate

Answer all questions and record all calculations in your lab book. 1. Calculate the appropriate amount of reagents necessary

Determine the optimal incubation condition for HaCat cells to grow?Preparation of H2O2 stock with media (positive control) 1. Calculate the volume of 1500 um H2O2 (stock) and media that is necPreparation of Matrix dilution 1. Calculate the volume of 10% matrix stock solution and media that is necessary to obtain fin

Answer all questions and record all calculations in your lab book. 1. Calculate the appropriate amount of reagents necessary to prepare a complete RPMI media using the following recipe. RPMI 1640 medium Starting concentration FBS 100% Non essential amino acids 10X 100U/ml penicillin and 10X streptomycin sulphate Concentration required 10% 1X 1X Complete RPMI (500ml)

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