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During a determination of the enzyme activity of a lactase enzyme with the model substrate ONPG, the spectrophotometer suddenly breaks down. Instead of measuring the
During a determination of the enzyme activity of a lactase enzyme with the model substrate ONPG, the spectrophotometer suddenly breaks down. Instead of measuring the absorbance of the chromophore ONP, the concentration of D-galactose is determined by HPLC (high pressure liquid chromatography). After 3 minutes, the enzyme reaction is stopped and the concentration of Dgalactose is measured at 0.217mg/ml in 600l total volume. What is the enzyme activity of the lactase enzyme? a) 0.160mol/min b) 0.362mol/min c) 0.402mol/min d) 0.241mol/min
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