For the following image (derived from PAS2.tif available in the UB Learns folder), if you have to train the computer to segment the nuclei, list the artifacts that you want the computer needs to remove for clean nuclei segmentation. Rank the artifacts that are needed to be removed with priority for efficient computation.

He wannna know what kinds of artifacts using matlab to make that dots ( Nuclei) more clear and remove the noise around them.

like convert this photo to binary image, knowing the intensity image of the background...etc what else I can do to remove the noise around that nuclei