Answered step by step
Verified Expert Solution
Question
1 Approved Answer
Hi, I need to assist with the lab questions 5. A student has a Bradford assay standard curve fit of y=1.11x +0.002. He has an
Hi, I need to assist with the lab questions
5. A student has a Bradford assay standard curve fit of y=1.11x +0.002. He has an unknown sample that he dilutes according to the table below. He subsequently conducts the Bradford assay on the diluted samples. + Complete the table below. a. Sample Sample volume (ul) Water volume (ul) Absorbance (595 nm) [protein (mg/ml) Ae le 99 0.003e Be 2e 98 0.011e ce 15e 85e 0.6682 De 1002 Oe 1.9852 b. Only one of the measurements is in range and should be used to calculate the protein concentration of his original unknown (concentrated) sample. Which one is it and why should he use this one? + 1 2 c. Calculate the concentration of the original unknown (concentrated) sampleHe diluted his sample 100 fold and added 100 ul of the diluted sample to the Bradford assay. The absorbance of the assay is 0.001. What does this indicate 5. A student has a Bradford assay standard curve fit of y=1.11x +0.002. He has an unknown sample that he dilutes according to the table below. He subsequently conducts the Bradford assay on the diluted samples. + Complete the table below. a. Sample Sample volume (ul) Water volume (ul) Absorbance (595 nm) [protein (mg/ml) Ae le 99 0.003e Be 2e 98 0.011e ce 15e 85e 0.6682 De 1002 Oe 1.9852 b. Only one of the measurements is in range and should be used to calculate the protein concentration of his original unknown (concentrated) sample. Which one is it and why should he use this one? + 1 2 c. Calculate the concentration of the original unknown (concentrated) sampleHe diluted his sample 100 fold and added 100 ul of the diluted sample to the Bradford assay. The absorbance of the assay is 0.001. What does this indicateStep by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started