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i just need help with part c only. help calculate Vmax, Km, and K2 1. Free (Non-inhibited) Reaction Enzyme Kinetics (30 points). An enzyme is
i just need help with part c only.
help calculate Vmax, Km, and K2
1. Free (Non-inhibited) Reaction Enzyme Kinetics (30 points). An enzyme is used to catalyze a oxidation reaction of AB according to Michaelis-Menten Kinetics. Data is given below: Table 1 X= [5] [5] ro X data Y data Estat (mol/dm) 3.0 3.0 3.0 3.0 3.0 3.0 S (mol/dm) 10 20 30 50 60 80 Te (mol/dmhour) 5.0 7.5 10.0 12.5 13.7 15.0 2.667 3 30 so 60 5.333 a. Prepare a Hanes-Woolf plot of the reaction by calculating the X-data and Y-data that would be used for the graph. You may put your values in the blank spaces in Table 1 above. Ho b. Draw the plot in the blank plot area below in Figure 1. + C. Evaluate the values of Vmax, K. and kz (the specific rate constant for the product generating step in the MM model) for the reaction in the given temperature. Report the values with the correct units. the 6.000 5.500 5.000 4.500 4.000 3.500 3.000 2.500 2.000 0 10 20 30 40 50 60 70 80 90 100 Figure 1. Draw the plot for Part B here: Table 2: Write your values for Part Chere. Vmax Slope = As 53-2 OR So - 1 -0.041 Vanaa 1 slope = 21.277 0.04 Int- Vamas -2 Km k2 too longe Units = hour ? 12.554 7.09 from Units Einterest is Units = am? to Jakulete (11= slope X 21.277 Etotal = Value Value = Value . \\ 0 5 0.333 3.0 mol/dm Step by Step Solution
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