In isocratic elution, the composition of the mobile phase is held constant during an LC paration. LC pumps should be able to generate pressures up to about 6000psi and flow rates between 0. 10mL/min. Common detectors for LC are UV-Vis and fluorescence spectrometers. A reverse-phase column has a polar stationary phase. In order to do the simulation exercise, I will need to bring a copy of the LC report form to lab