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Inosine nucleosidase catalyzes the hydrolysis of inosine monophosphate to hypoxanthine and ribose phosphate. Enzyme activity is determined by measuring initial rates of hydrolysis of inosine
Inosine nucleosidase catalyzes the hydrolysis of inosine monophosphate to hypoxanthine and ribose phosphate. Enzyme activity is determined by measuring initial rates of hydrolysis of inosine monophosphate. Starting with a inosine monophosphate concentration CS0=33mM, the following results have been obtained: Draw the profile of substrate concentration across time. Determine the kinetic parameters of the enzyme using the linearization method of Lineweaver-Burk. The correctness of the fitting to experimental data should be confirmed by calculating Determination Coefficient (R2), Root Mean Square Errors (RMSE) and Sum of Squared Errors (SSE). Give a graphical interpretation of the method used
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