John working in a biopharmaceutical company and is in charge of purifying Insulin (Mw = 6,000 Da, pI =7.3). In the recent experiment, the insulin was contaminated by a glycoprotein (Mw=48,000 Da, pI= 6.9) and protein A (Mw=5,500 Da, pI = 10.2). Please help John to design a process based on chromatography methods to purify his product.

If John run his contaminated products on a sodium dodecyl sulfate-polyacrylamide (SDS-PAGE), gel, please draw the represented picture gel. Please also draw the protein elution plot if this mixture is separated using size exclusion chromatography