Lab: NO \& macrophages in protection from TB Expecting just two items for task 1 using results from Nitric Oxide Results 2021 Updated (on BM214) 1. Standard curve. Use the data in yellow. The nitrite concentrations used are identical to the first standard curve. 2. Table: showing all the results for the samples (nitrite levels in M ) for task 1 - using data from all 5 plates shown in Nitric Oxide Results 2021. Use the pattern set up for P5/U5 to automate this - the new curve should not be used. Calculate the mean nitrite concentrations (M)SD for control and antigen stimulated samples. We have provided information on the results for task 2. Need legends and statistics shown on the Table (No need to prepare chart as well) Task 2: 'Task 2 information - effect of neutralising antibodies' on BM214 page. How do the Ab affect NO Production - what can you infer from that? This should be included in your discussion but you do not have to present this data in the results. \begin{tabular}{|l|l|l|l|l|l|l|l|l|} \hline & \multicolumn{1}{|l|}{ DILUTION 1 } & DILUTION 2 & UNSTIM U3 & STIMU3 ANTIGEN & CON AU3 & UNSTIM P 3 & STIM P3 ANTIGEN & STIM P3 CONA \\ \hline A & & & & & & & & \\ \hline B & 0.336 & 0.339 & 0.0495 & 0.0728 & 0.3201 & 0.0873 & 0.1397 & 0.2192 \\ \hline C & 0.116 & 0.196 & 0.0485 & 0.0757 & 0.3153 & 0.1164 & 0.1465 & 0.2386 \\ \hline D & 0.075 & 0.077 & 0.0514 & 0.0757 & 0.3162 & 0.1649 & 0.1445 & 0.2813 \\ \hline E & 0.056 & 0.055 & & 0.0757 & 0.3007 & 0.1358 & 0.1455 & 0.2444 \\ \hline F & 0.044 & 0.048 & & & & & & \\ \hline G & 0.039 & 0.047 & & & & & & \\ \hline H & 0.034 & 0.038 & 0.3300 & 0.3300 & & & & \\ \hline \end{tabular} U2 P2 Absorbance Note layout includes only part of a task 2 plate. Your standard curve will also be in a different location ABCDEFGH10.35940.16740.11970.08170.04880.04250.03340.036320.36610.20350.12120.07830.04710.05210.03320.037730.0570.05240.05820.05580.03120.03270.03120.272540.05220.05240.05210.05460.03190.03240.03030.027450.32840.43270.51050.34830.03490.03260.03040.030460.10290.09120.09050.090.03110.03490.03060.035870.20260.18340.18690.18760.03120.03320.03520.03180.22280.23270.2090.22470.03140.03250.03320.030990.02890.03040.03090.03160.03930.03220.03030.0312100.02960.02970.03240.03350.03230.03480.03450.0311110.02810.03030.03030.03120.03240.03150.03120.0314120.02860.02860.03330.03270.03120.03560.03130.0376 Chart Title \begin{tabular}{lrrrrrrrrrrrrrrrr} U2P2 & Nitrite levels & \multicolumn{2}{c}{L} & \multicolumn{2}{c}{ Only IFN Ab shown } \\ & 1 & 2 & 3 & 4 & 5 & 6 & 7 & 8 & 9 & 10 & 11 & 12 \\ A & 0.359 & 0.366 & 7.273 & 5.818 & 89.515 & 21.182 & 51.394 & 57.515 & 1.24242 & 1.0303 & 1.48485 & 1.33333 \\ B & 0.167 & 0.204 & 5.879 & 5.879 & 121.121 & 17.636 & 45.576 & 60.515 & 0.78788 & 1 & 0.81818 & 1.33333 \\ C & 0.120 & 0.121 & 7.636 & 5.788 & 144.697 & 17.424 & 46.636 & 53.333 & 0.63636 & 0.18182 & 0.81818 & 0.090909 \\ D & 0.082 & 0.078 & 6.909 & 6.545 & 95.545 & 17.273 & 46.848 & 58.091 & 0.42424 & 0.151515 & 0.54545 & 0.09091 \\ E & 0.0488 & 0.0471 & 0.54545 & 0.33333 & 0.575758 & 0.57576 & 0.54545 & 0.48485 & 1.909091 & 0.21212 & 0.18182 & 0.54545 \\ F & 0.0425 & 0.0521 & 0.09091 & 0.18182 & 0.12121 & 0.575758 & 0.060606 & 0.15152 & 0.24242 & 0.545455 & 0.45455 & 0.787879 \\ G & 0.0334 & 0.0332 & 0.54545 & 0.81818 & 0.78788 & 0.72727 & 0.666667 & 0.060606 & 0.81818 & 0.454545 & 0.54545 & 0.51515 \\ H & 0.0363 & 0.0377 & 72.57576 & 1.69697 & 0.78788 & 0.848485 & 0.60606 & 0.63636 & 0.54545 & 0.57576 & 0.48485 & 1.393939 \end{tabular} Mean nitrit 6.9246.008112.72018.37947.61457.364