A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An aliquot was removed from the reaction mixture every minute for 5 minutes
A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained.
Time (min) A260
0 0.60
1 0.64
2 0.67
3 0.70
4 0.72
5 0.73
Describe the action of deoxyribonuclease on DNA and explain the increase in A260.
Step by Step Solution
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There are 3 Steps involved in it
Step: 1
DNase is the enzyme which helps to cleave the DNA into the nucleotide bases As experiment is done wi...
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Step: 2
Step: 3
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Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
4th edition
470547847, 978-0470547847
