Question
Ocean pout, a fish found in cold waters, produce an antifreeze protein that allows them to survive at near freezing temperatures. Your company plans to
Ocean pout, a fish found in cold waters, produce an antifreeze protein that allows them to survive at near freezing temperatures. Your company plans to genetically engineer bacteria to grow antifreeze proteins to improve the storage of ice cream products
a) The vector and PCR product that you will use to clone the antifreeze gene are shown above along with the locations of three different restriction enzyme cut sites Which restriction enzyme (Hindill, BamH1, or EcoR1) will allow you to successfully clone this transgeno? Brietly justify your answer.
b) Describe how you will select for bacteria that carry the recombinant DNA molecule once you have completed the transformation
Hindil lact EcoR1 Promoter Coding region GFP EcoRI HamHI BamHI
Step by Step Solution
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1 The latter are type ii endonucleases that are used to cut DNA at palindromic sites for transformat...
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Elementary Statistics
Authors: Mario F. Triola
12th Edition
0321836960, 978-0321836960
