Only answer questions 3 and 5. You will need information from question 4 to answer.

3. A batch of pharmaceuticals was sterilized by a method that results in a 6-log reduction. A follow-up assay found 1% contamination. What is the initial contamination level? 1. (a) 10 2. [b] 10 3. (C 102 4. Idj 10 5. (e] 1 4. A chemical sterilization procedure is found to destroy the indicator organism B. subtilus with D-value of 23 min. What is the rate constant of cell killing? 1. (a) 10 min 2. (bj 10h1 3. [C] 10 s11 4. Idj 1 min 5. (e] None of the above 5. Which of the following is a reasonable D-value for non-indicator E. coli subjected to the same procedure in Question 4? 1. (a) 23 days 2. jbj 23h 3. [b] 2.3 h 4. icj 23 min 5. (e) 2.3 min