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Part 1: Gram Stain Procedure Procedure 1. Examine the finished slides under a microscope using the 10X, 40X, and the oil immersion (100X) lenses
Part 1: Gram Stain Procedure Procedure 1. Examine the finished slides under a microscope using the 10X, 40X, and the oil immersion (100X) lenses to determine whether each specimen is Gram-positive or Gram-negative. 2. Draw representative cells from each organism. Be sure to note differences in cellular morphology (size, shape, arrangement, etc.). 3. Complete the table. Indicate the name of the organism, its morphology (cell shape and arrangement), the color, and whether it is Gram-positive or Gram-negative. You may refer to Exercise 3 for a review of bacterial cellular morphology. 4. When finished discard the slides in the biohazard bin and return all stock cultures to your instructor. Results and Observations Staphylococcus aureus Proteus vulgaris Escherichia coli Enterobacter aerogenes Bacillus subtilis Streptococcus lactis S. aureus 1000x Total Magnification 77 KI E. coli 1000x Total Magnification Mo 3.1 1000x Total Magnification T P. vulgaris 1000x Total Magnification P. vulgaris 1000x Total Magnification E. aerogenes 1000x Total Magnification 946 S. lactis 1000x Total Magnification Organism Cellular Morphology and Arrangement Staining Color Gram Reaction
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