Answered step by step
Verified Expert Solution
Question
1 Approved Answer
pH needs to be 7.1 Make me a phosphate buffer with a given p H value (see below) so I can study some blood proteins.
pH needs to be 7.1
Make me a phosphate buffer with a given p H value (see below) so I can study some blood proteins. The concentration of ions in blood (it's ionic strength) is in the neighbourhood of 150mM.1 Make me enough so that I can have at least 1000 samples of 2mL each. Data: pKa values for phosphoric acid, H3PO4, are 2.1,7.2 and 12.3 You have three bottles of crystalline solids: NaH2PO4, mw 120g/mole;Na2HPO4, mw 142g/mole;Na3PO4, mw 164g/mole. I spent the big bucks on anhydrous salts for you. As you do your calculations please consider what properties are desirable in a biological buffer Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started