Please andwer questions 4 and 8.

PreLab Questions: 1. What is a database? What ipformation goes PBD contain? (Points 1+1 ) - A public re osito of of targe Yolumes of in formation or data about a particulat Yopic or data about a particular topic - structures of all macromolecules 2. Which of the two (PDB and UniProt) will be a larger database? (Points 1) - Uniprot 3. What are splice variants of a gene? Given a protein, how will you know if it has splice variants? (Points 1+1) mRNA is di fferent in the same gene of different cells. - use Uniprot and go to cross-reference's section to sce splice variants 4. How will one know how many polypeptide chains are present in the quaternary structure of a protein molecule? (Points 1) 5. In a BLAST search, which of the two E-values (104 and 107) will provide higher confidence in selecting a homolog? (Points 1) 107 6. Why is structural alignment considered superior to a sequence alignment? (Points 1) Structural alighment shows the chancye in the structure due to the changy in sequenele 7. Will the results of the BLAST search depend on the database selected? (Points 1) 8. How does one know how many amino acids a protein contains? (Points 1 ) Experiment 5 Bioinformatics (1): Introductory Lab for Computational Biochemistry 53