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Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find H, S and

Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find H, S and G(T=75) (mention any assumptions you made) and What is the Tm for each protein.

RNase A

Temperature C Absorbance
40 0.126
50 0.125
60 0.122
65 0.137
70 0.138
75 0.143
80 0.185
90 0.197

Urea Denaturation
M urea Absorbance
1 0.112
2 0.097
4 0.088
6 0.131
8 0.14

T 1/T K
40 0.025 -0.7520811
50 0.02 -0.6820811
60 0.01666667 -0.6850811
65 0.01538462 -0.6700811
70 0.01428571 -0.6690811
75 0.01333333 -0.6640811
80 0.0125 -0.6220811
90 0.01111111 -0.24

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