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Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find H, S and
Please help me Calculate fraction denatured and equilibrium constant at each temperature and plot ln K vs T-1 graph, once plotted Find H, S and G(T=75) (mention any assumptions you made) and What is the Tm for each protein.
RNase A
Temperature C | Absorbance |
40 | 0.126 |
50 | 0.125 |
60 | 0.122 |
65 | 0.137 |
70 | 0.138 |
75 | 0.143 |
80 | 0.185 |
90 | 0.197 |
Urea Denaturation | |
M urea | Absorbance |
1 | 0.112 |
2 | 0.097 |
4 | 0.088 |
6 | 0.131 |
8 | 0.14 |
T | 1/T | K |
40 | 0.025 | -0.7520811 |
50 | 0.02 | -0.6820811 |
60 | 0.01666667 | -0.6850811 |
65 | 0.01538462 | -0.6700811 |
70 | 0.01428571 | -0.6690811 |
75 | 0.01333333 | -0.6640811 |
80 | 0.0125 | -0.6220811 |
90 | 0.01111111 | -0.24 |
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