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Pseudomonas aeruginosa strain which is able to convert H g + 2 into elemental H g 0 , is isolated from sediment in a river.

Pseudomonas aeruginosa strain which is able to convert Hg+2 into elemental Hg0, is
isolated from sediment in a river. The bacteria are to be utilized for onsite
bioremediation of soil contaminated with methyl mercury, which is nontoxic to humans.
Kinetic parameters for the organism are found using data obtained from chemostat
culture. A 1-L fermenter is used with a feed stream including 100M of methyl mercury.
Steady state substrate concentrations are measured as a function of chemostat flow rate.
a. Calculate max and Ks of the organism.
b. Calculate the maximum practical operating flow rate.
Not: The plot should be obtained both by hand and Excel. All results should be
attached to homework. All the calculated data should be shown on a table.
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