Q1. What is the protein concentration in mg/mL of the unknown protein solution (allow for the dilution used)? Which dilution(s) did you use for your calculations and why?

8 9 10 11 12 Tube Unknown, mL 7 1.0 1.0 - 1.0 1.0 1.0 1.0 1in 3 dilution, mL 1in 9 dilution, mL Final volume/tube, mL 1.0 1.0 1.0 1.0 1.0 1.0 0.5676 0.5661 0.2186 0.2091 0.0782 0.0710 1.802866 1.798137 0.70274 0.672794 0.260169 0.237473 Absorbance (540 nm) Unknown [protein] (mg/mL) Mean unknown protein (mg/mL) 1.800501 0.687767 0.248821 0.35 0.3 0.25 0.2 Absorbance (540 nm) 0.15 0.1 0.05 0 0 0.2 0.4 0.6 0.8 1 1.2 Protein concentration mg/mL Figure 1: Standard curve for protein determination 8 9 10 11 12 Tube Unknown, mL 7 1.0 1.0 - 1.0 1.0 1.0 1.0 1in 3 dilution, mL 1in 9 dilution, mL Final volume/tube, mL 1.0 1.0 1.0 1.0 1.0 1.0 0.5676 0.5661 0.2186 0.2091 0.0782 0.0710 1.802866 1.798137 0.70274 0.672794 0.260169 0.237473 Absorbance (540 nm) Unknown [protein] (mg/mL) Mean unknown protein (mg/mL) 1.800501 0.687767 0.248821 0.35 0.3 0.25 0.2 Absorbance (540 nm) 0.15 0.1 0.05 0 0 0.2 0.4 0.6 0.8 1 1.2 Protein concentration mg/mL Figure 1: Standard curve for protein determination