question: predict the order of amino acids in terms of highest Rf value. The amino acids are shown in the picture with their 1 letter abbreviation.

You will be using a mixture of 5:1:4 volume ratio of butanol, glacial acetic acid (pure acetic acid), and water in this lab. Assume that butanol is the same as water in terms of a pH calculation. The density of acetic acid is 1.05g/ml. The molecular weight of acetic acid is 60.05g/mol. The Ka of acetic acid HAc is 1.8105. You can make the assumption that [H+]=[Ac]