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The figure above shows light passing first through a specimen, then through the glass cover slip over the specimen, through a medium of index of
The figure above shows light passing first through a specimen, then through the glass cover slip over the specimen, through a medium of index of refraction n and finally entering the microscope lens. Assume the medium is air (let n = 1.00). The index of refraction of the glass slip is 1.54. (a) If the angle 0 of light hitting the coverslip-air interface is 38 degrees, what is the NA for the microscope lens under these conditions? microscope tube D - lens L coverslip microscope slide (b) What is the smallest distance that can be resolved with light of wavelength 550 nm in the set-up above? (c) NA of an objective determines the resolution of the lens. How can one increase NA of the objective lens? List at least two factors
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