Question
The SDS-PAGE gel below contains 3 lanes in which samples can be separated using an electric charge. Draw the results of the separation of the
The SDS-PAGE gel below contains 3 lanes in which samples can be separated using an electric charge. Draw the results of the separation of the following samples: a. Lane 1 contains a purified heterodimer with intermolecular disulfide bonds. The polypeptides have molecular weights of 50 and 65 kDa (Da = Dalton = g/mol). b. Lane 2 contains the same purified heterodimer as lane 1 with the addition of - mercaptoethanol (BME). c. Lane 3 contains protein standards of known molecular weight: 200, 120, 100, 80, 60, 40, 20 kDa. Label each of the pre-drawn bands.
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