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This is a Scatchard plot for the formation of protein-ligand complex where there is only one binding site. The protein concentration is 3.1M. The data

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This is a Scatchard plot for the formation of protein-ligand complex where there is only one binding site. The protein concentration is 3.1M. The data at 20C is in black and the data at 37C is in red (with dashes). Based on the plot above, determine the dissociation constant (in M ) at: 20C 37C

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