The same protein lysates were also probed with antibodies against Ire1 and Hac1 (also known as XbpP1), along with anti-actin. Which blot (right or left) was labeled with anti‐Ire1? Which blot (right or left) was labeled with Hac1 (Xbp1)? How do you explain the presence of a ~30 kDa band in juniferdintreated cells but not in untreated cells in the blot on the right? before dialysis after dialysis 1 + 2 ‐1 + 3 ‐ + 2 + 3 ‐transcriptional activity samples mixed 4c. Immunocytochemistry and fluorescence microscopy analysis was carried out using the antibody utilized in a blot on the right (in question 4b) and detection was achieved using a secondary antibody labeled with rhodamine (red). Since juniferdin specifically affects PDI, a resident rough ER protein, how do you explain the localization of the signal in the nucleus?