Question
To determine the identity of an unknown tyrosinase inhibitor, you run two sets of rate experiments (one contains only enzyme and substrate and no inhibitor
To determine the identity of an unknown tyrosinase inhibitor, you run two sets of rate experiments (one contains only enzyme and substrate and no inhibitor while the other set contains enzyme, substrate and fixed amount of inhibitor). Based on the line of equation obtained from the Lineweaver Burk plot for these two sets of rate experiments, which of the following compounds is most likely the unknown inhibitor?
Enzyme + substrate only: y = 45.36x + 16.279 Enzyme + substrate + inhibitor: y = 115.97x + 16.278
4-methoxybenzoic acid (p-anisic acid) | ||
Sodium benzoate | ||
Dopachrome | ||
None of the above |
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