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Using only Blackstrap molasses and corn steep liquor, formulate a medium for growth of Saccharomyces cerevisiae to a density of 2 0 gm / L
Using only Blackstrap molasses and corn steep liquor, formulate a medium for growth of Saccharomyces cerevisiae to a density of gmL dry wt with the simultaneous synthesis of ethanol to a final concentration of gmL Assume that Yxglucose gm dry wt cellsgm glucose; YEtOHglucose is of the maximum theoretical value, and that Ms gm glucosegm dry wt cellsh Assume that the yields for growth and product formation are the same for fructose, sucrose, and glucose. Also assume that all protein and amino acid nitrogen is available for use by the yeast. Satisfy the cellular demand for carbon and nitrogen in the medium formulation and check to see if the medium formulated on this basis meets the
need for sulfur and phosphorus. Assume that the maximum biomass density is obtained in h exponential growth with a population doubling time of h and that the entire process growth and product formation takes h
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