Which is the best assay? Use the knowledge you've gained through the course of this exercise to determine which of three test conditions will yield the best results for a hypothetical RIA system that you are trying to develop. You have been given only raw data; pay attention to how they are labeled (i.e. standards, parallel, NSB, etc.) and carefully look at the numbers. If you are comfortable with what you've done there is a clear winner. Condition 1 Condition 2 Condition 3 CPM 1 CPM 2 CPM 3 CPM 1 CPM 2 CPM 3 CPM 1 CPM 2 CPM 3 Sample Num Sample ID 1 Total Activity 2 Blank 51233 50956 51564 50956 51123 50123 50456 50621 50101 16841 3546 3641 3550 16589 16153 12024 20340 16527 9987 9545 9874 1201 1097 1246 854 1121 1489 402 412 398 1204 1198 1265 957 1056 1645 1104 1098 1245 3012 2975 2899 3894 3219 2140 1987 2010 1954 5123 5241 5278 3241 6541 5780 2654 2547 2589 7456 7412 6897 5148 9994 7396 9987 3125 3256 2998 10103 10057 8879 11112 15893 3826 3798 3845 12456 12631 12632 15439 8158 11478 493 451 468 998 1001 1098 2156 1689 1045 1654 1598 1675 2458 2514 2398 3511 2545 4856 2014 2089 2101 4500 4489 4512 7210 4671 5486 3014 3102 3069 7214 7245 7198 5489 10120 8456 3 NSB 4 Standard 1 - 1000 5Standard 2 - 500 6 Standard 3 - 250 7 Standard 5 - 100 8 Standard 6-50 9 Standard 7 - 20 10 Parallel - 1:2 11 Parallel - 1:4 12 Parallel - 1:8 13 Parallel - 1:16 14 Parallel - 1:32 15 Parallel - 1:64 16 Recovery Background 17 Recovery 1000 18 Recovery 500 19 Recovery 250 20 Recovery 100 21 Recovery 50 22 Control Pool 1 23 Control Pool 2 24 Control Pool 3 3255 3311 3284 10024 10098 9999 6479 11481 14520 3312 3295 3254 12321 12287 12301 12045 14521 16362 4475 4511 4485 12456 12897 12569 11456 13879 10099 789 721 745 1045 1121 1098 1154 998 1324 1387 1399 1445 3145 3098 3450 2987 2741 2010 2145 2000 4923 3077 4987 7451 5010 2897 4412 7245 2999 3020 2845 7500 7498 7523 8012 6470 3600 3589 3475 8989 9020 9001 7412 8650 9974 1450 1398 1421 3541 3621 3145 3245 3952 2863 2085 2141 2174 5600 5589 5665 2504 5645 7641 3059 3101 3096 8102 8009 8045 9014 7683 7045 Which is the best condition? Why? Which is the best assay? Use the knowledge you've gained through the course of this exercise to determine which of three test conditions will yield the best results for a hypothetical RIA system that you are trying to develop. You have been given only raw data; pay attention to how they are labeled (i.e. standards, parallel, NSB, etc.) and carefully look at the numbers. If you are comfortable with what you've done there is a clear winner. Condition 1 Condition 2 Condition 3 CPM 1 CPM 2 CPM 3 CPM 1 CPM 2 CPM 3 CPM 1 CPM 2 CPM 3 Sample Num Sample ID 1 Total Activity 2 Blank 51233 50956 51564 50956 51123 50123 50456 50621 50101 16841 3546 3641 3550 16589 16153 12024 20340 16527 9987 9545 9874 1201 1097 1246 854 1121 1489 402 412 398 1204 1198 1265 957 1056 1645 1104 1098 1245 3012 2975 2899 3894 3219 2140 1987 2010 1954 5123 5241 5278 3241 6541 5780 2654 2547 2589 7456 7412 6897 5148 9994 7396 9987 3125 3256 2998 10103 10057 8879 11112 15893 3826 3798 3845 12456 12631 12632 15439 8158 11478 493 451 468 998 1001 1098 2156 1689 1045 1654 1598 1675 2458 2514 2398 3511 2545 4856 2014 2089 2101 4500 4489 4512 7210 4671 5486 3014 3102 3069 7214 7245 7198 5489 10120 8456 3 NSB 4 Standard 1 - 1000 5Standard 2 - 500 6 Standard 3 - 250 7 Standard 5 - 100 8 Standard 6-50 9 Standard 7 - 20 10 Parallel - 1:2 11 Parallel - 1:4 12 Parallel - 1:8 13 Parallel - 1:16 14 Parallel - 1:32 15 Parallel - 1:64 16 Recovery Background 17 Recovery 1000 18 Recovery 500 19 Recovery 250 20 Recovery 100 21 Recovery 50 22 Control Pool 1 23 Control Pool 2 24 Control Pool 3 3255 3311 3284 10024 10098 9999 6479 11481 14520 3312 3295 3254 12321 12287 12301 12045 14521 16362 4475 4511 4485 12456 12897 12569 11456 13879 10099 789 721 745 1045 1121 1098 1154 998 1324 1387 1399 1445 3145 3098 3450 2987 2741 2010 2145 2000 4923 3077 4987 7451 5010 2897 4412 7245 2999 3020 2845 7500 7498 7523 8012 6470 3600 3589 3475 8989 9020 9001 7412 8650 9974 1450 1398 1421 3541 3621 3145 3245 3952 2863 2085 2141 2174 5600 5589 5665 2504 5645 7641 3059 3101 3096 8102 8009 8045 9014 7683 7045 Which is the best condition? Why
