Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from a CATION EXCHANGE column with carboxymethyl groups in the stationary phase and a mobile phase with pH = 6.0. Each correctly assigned choice (i.e., either checked or not) earns 0.25 marks. Each incorrectly assigned choice earns 0 marks.

Question 10 options:

	Increasing the pH of the mobile phase from 6.0 to 7.0 AND increasing the concentration of salt (NaCl) from 0.1 M to 0.5 M for a protein with pI = 7.5.
	Decreasing the pH of the mobile phase from 6.0 to 5.0 for a protein with pI = 6.5.
	Increasing the concentration of salt (NaCl) in the mobile phase from 0.1 M to 0.5 M for a protein with pI = 7.0.
	Increasing the pH of the mobile phase from 6.0 to 7.0 for a protein with pI = 6.5.