Answered step by step
Verified Expert Solution
Question
1 Approved Answer
You are working in a molecular biology lab and have been given four oligonucleotides (short fragments of DNA) that all contain 20 bases (see sequences
You are working in a molecular biology lab and have been given four oligonucleotides (short fragments of DNA) that all contain 20 bases (see sequences below). You are given a solution of each oligo at a concentration of 100M (based on the mass of the DNA and the calculated molecular weight of the sequence). Oligo A 5-GTGACCAATCGATTGGTCAC-3 Oligo B 5-GGAGACAAGGTCGGAGGAAT3 Oligo C 5-GTGACCAATCGATTGGTCAC3 Oligo D 5-CCCTACGTGCAAGGCATGCG3 Q1.1 Absorbance at 260nM 2 Points The concentration of a solution of DNA can be determined by measuring the absorbance of the solution at 260nM(A260) and multiplying by 50g/ml. When you measure the A260 of each your oligonucleotide solutions, one of them appears to have a higher absorbance than the others, even though they all have the same initial concentration. Which one of the four oligos is it and explain why it would absorb more light at 260nM
Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started