1. 2. 3. 0.5 mL is added to 9.5 mL of diluent. 100 WBCs are counted in four of the 1 mm squares of a Neubauer hemacytometer. Dilution Area Counted Depth WBC Count cells/uL or Note: When expressing cell counts in units that require an exponent, round off your answer to the nearest tenth. Example: 5,460 cells = 5.5 X 10 cells/uL = 5.5 X 10' cells/L Depth WBC Count (units in millimeters) (units in millimeters) 1 mL of specimen is added to 9 mL. 180 WBCs are counted in all 9 squares. Dilution Area Counted Area Counted Volume WBC Count X 10 cells/uL or (units) (units) cells/uL or 0.4 uL of blood is added to 9.6 uL of diluent. 100 cells are counted in four of the 1 mm squares. Dilution (units) (units) cells/uL or X 10 cells/L X 10 cells/uL or X 10' cells/L X 10 cells/uL or X 10 cells/L 4. 5. 6. 7. 0.1 uL of blood is diluted with 0.9 uL of diluent. 200 cells are counted in 9 -1 mm squares. Dilution Area Counted WBC Count Dilution 0.05 uL of blood is added to 0.95 uL of diluent. 100 cells are counted in 8 large squares (4 squares on each side of hematocytometer). Area Counted WBC Count (units) cells/uL or Dilution (units) cells/uL or Area Counted Depth Absolute Eosinophil Count X 10 cells/uL or X 10' cells/L 600 cells are counted in 4 large mm squares on a 1:10 dilution. WBC Count cells/uL or X 10 cells/uL or (units) (units) X 10 cells/uL or In performing an Eosinophil count in which the dilution is 1:16, 432 cells are counted in 18 squares, using both sides of the hemacytometer. X 10 cells/L cells/uL X 10 cells/uL or X 10 cells/L X 10 cells/L 8. 50 WBC cells are counted in a body fluid, diluted 1:20, and 4 square mm are counted on the hemacytometer. Name two (2) ways to increase the accuracy of this count.