1. You decide to look for protein binding partners of Fisk-250 and discover that Meharry-250 binds to Fisk-250 (YAY!!). Design an experiment that tests whether Meharry-250 is critical to Fisk-250 FUNCTION given data in 2b and 2c.

2b. 2c.

120 100 80 Percent cells 60 40 20 0 Control siRNA Fisk-250 siRNA 1 GO IS G1 M Results from RNA- Sequencing experiments 350 300 Relative mRNA levels 250 200 150 100 50 0 2 p21 Kras GAPDH Control siRNA Fisk-250 siRNA 120 100 80 Percent cells 60 40 20 0 Control siRNA Fisk-250 siRNA 1 GO IS G1 M Results from RNA- Sequencing experiments 350 300 Relative mRNA levels 250 200 150 100 50 0 2 p21 Kras GAPDH Control siRNA Fisk-250 siRNA