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You want to test the binding of a novel protein (NP) on a surface bound cellular receptor. You isolate 0.25mg of cell-membrane and incubate it
You want to test the binding of a novel protein (NP) on a surface bound cellular receptor. You isolate 0.25mg of cell-membrane and incubate it with increasing concentrations of NP. At a concentration of 0.03M and above you measure the amount bound to the membrane at 1.2pmol. You find that 0.6pmol is bound to the cell-membrane at NP concentration of 0.007M. A) What are the values of TR and kc for this novel protein? B) What percentage of receptors would be bound if the NP cencentration was 0.0035M
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