Suppose you have two genetic variants of a large protein that dier only in that one contains

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Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pKa = 6.0) when the other has a valine (uncharged side chain).
a. Which would be better for separation: gel electrophoresis or isoelectric focusing? Why?
b. What pH would you choose for the separation?
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Biochemistry Concepts and Connections

ISBN: 978-0321839923

1st edition

Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews

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