You have discovered a novel protein that has a pI = 5.5. To study the functional properties

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You have discovered a novel protein that has a pI = 5.5. To study the functional properties of this new protein, your research group has made a mutant that contains two amino acid changes-namely, a surface Phe residue in the normal protein has been replaced by His (side chain pKa = 6.1), and a surface Gln has been replaced by Glu (side chain pKa = 6.0). Is the pI of the mutant protein predicted to be greater than, less than, or the same as the pI of the normal protein? Support your answer with the appropriate calculation.
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Biochemistry Concepts and Connections

ISBN: 978-0321839923

1st edition

Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews

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