Question: T7 DNA polymerase is less sensitive to dideoxynucleotides than the E. coli DNA polymerase I fragment described in Section 3-4C. (a) For a given concentration
T7 DNA polymerase is less sensitive to dideoxynucleotides than the E. coli DNA polymerase I fragment described in Section 3-4C.
(a) For a given concentration of dideoxynucleotides, which enzyme is more likely to give a longer "ladder" on the sequencing gel?
(b) Which enzyme is best used for sequencing DNA close to the primer?
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