The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data

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The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows:

a. Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting clear answers?
b. Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?

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Biochemistry Concepts and Connections

ISBN: 978-0321839923

1st edition

Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews

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Question Posted: Apr 21, 2016 04:15 AM

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The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data

Question:

[S] (μ mol/L) v[(μ mol/L) min 1) 10 20 50 39 65 102 120 135 100 iki 200

Step by Step Answer:

a It is difficult to estimate V max this way A value of about 150 ...View the full answer

Biochemistry Concepts and Connections

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