To carefully prepared mitochondria were added succinate, oxidized cytochrome c, ADP, orthophosphate, and sodium cyanide. Referring to Figure 14.16, answer the following.
(a) List the sequence of electron carriers in this system.
(b) Write a balanced equation for the overall reaction occurring in this system, showing oxidation of the initial electron donor, reduction of the final acceptor, and synthesis of ATP.
(c) Calculate ˆ†G°€² for the overall reaction. ˆ†G°€² for ATP hydrolysis is - 32.2 kJ / mol.
(d) Why was cyanide added in this experiment?

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P/O 1.5 Succinate Ascorbate Rotenone and amytalFAD inhibit TMPD Cyanide, azide, and carbon monoxide inhibit complex IV by reaction with cytochrome a B-Hydroxybutyrate complex I AntimycinA NAD FeCoacyt b Fe-S cyt a3 FMN cyt c cyt c cyt a Ferricyanide Ferricyanide Exogenous cytochrome c Coupling site Complex I Coupling site Complex IlI Coupling site Complex IV