Question: You have several cell lysates and want to determine which of them contain protein X in its phosphorylated form (with phosphorylation occurring on a tyrosine
You have several cell lysates and want to determine which of them contain protein X in its phosphorylated form (with phosphorylation occurring on a tyrosine residue). You have two antibodies: One recognizes phosphotyrosine and the other recognizes protein X. Describe how you would analyze the cell lysates for the presence of phosphorylated protein X using just these two antibodies.
Step by Step Solution
3.39 Rating (168 Votes )
There are 3 Steps involved in it
Immimoaffinity chromatography immunoassay and Western blotting could wor... View full answer
Get step-by-step solutions from verified subject matter experts
Document Format (1 attachment)
930-M-S-B-C (1053).docx
120 KBs Word File
